生物反应器灭菌：你需要知道什么

Written by:亚瑟特拉帕多斯

Bioreactors — vessels designed to promote the growth of organisms such as yeast, bacteria or animal cells under controlled conditions — are a critical component in biotechnology companies, and a common device used in both established firms and startups. In order to prevent contamination, which can be incredibly costly in by time lost and money spent, it’s important to properly sterilize bioreactors between uses.

Although there are multiple different methods of sterilizing a bioreactor, steam sterilization is the most common — and the most effective. Here are a few things you should know about sterilizing a bioreactor using an autoclave.

Can My Bioreactor Fit in the Autoclave?

频繁，生物技术的设施误认为是他们现有的高压釜足够大以容纳生物反应器，或者他们在不采取生物反应器的尺寸考虑的情况下购买高压灭菌器。例如，来自特定制造商的生物反应器必须以特定的方向装入高压釜中，或者必须在特定的二级容器结构上装载。换句话说，您可能无法在垂直位置加入高压灭菌器。

Also, depending on its size and weight, a bioreactor may be far easier to maneuver into the autoclave when placed on a wheeled loading cart and carriage rather than on stationary shelves within an autoclave. To avoid any complications, be sure to talk to an autoclave specialist about the dimensions of any bioreactors you may need to sterilize.

我对我的生物反应器使用哪个灭菌循环？

Typical bioreactors are large heat-resistant containers that do not usually have sensitive components, which means the primary concern for sterilization is ensuring that there are no air pockets trapped within the container itself. A真空预循环is usually the best way to ensure that air can escape the bioreactor prior to sterilization taking place, as well as following any and all manufacturer guidelines with regards to positioning and partial or full disassembly of the bioreactor within the autoclave. Manufacturers of such specialized equipment will often have highly specific guidance when it comes to sterilization procedures, and this guidance should always be followed.

某些生物反应器需要专门的高压釜循环。例如，交替的切向流动（ATF）生物反应器使用中空纤维过滤器通过温度或压力的快速变化易于损坏。因此，ATF生物反应器需要灭菌循环gradually increases temperature and pressure, allowing these gradients to equalize across the filter. Properly sterilizing ATF bioreactors require a number of software and hardware customizations as well.

In order to avoid any potential damage, be sure to consult an autoclave specialist about which sterilization cycle to use for your specific type of bioreactor (or bioreactors, as the case may be).

Other Key Considerations

For organizations such as a biotechnology incubator space or a shared lab facility that house multiple different companies conducting different types of research, it may make sense to acquire an array of autoclaves to accommodate the various types and sizes of bioreactors that require sterilization.

如果您对您的生物反应器使用了什么类型的高压灭菌或灭菌循环有任何其他问题，talk to the experts在综合灭菌系统。我们有经验为世界上一些领先的生物技术公司和孵化器提供高压灭菌器

亚瑟特拉帕多斯

Arthur has over 20 years of experience in the sterilizer industry and possesses a background in biotechnology, chemical engineering, and business management. He regularly presents at conferences around the country about eco-friendly autoclave design features, as well as emerging trends in the steam sterilizer market. Arthur graduated from Tufts University with a B.S. and M.S. in Biochemical Engineering and earned an M.B.A. in Entrepreneurship from Babson College. Although he dedicates much of his focus to driving the needle of autoclave innovation forward, Arthur is also a strong advocate for leading a balanced lifestyle of work, family and fitness — an outlook he shares with all of his employees.

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